FIG 5.

DECR is required for β-oxidation of linoleic acid in vivo. (A) L. major promastigotes were labeled with ¹³C-labeled linoleic acid and levels of selected ¹³C-FAs determined as methyl esters by GC/MS. ¹³C-labeled linoleic acid is incorporated into parasites. Shown are means with standard errors of the means (SEM) representing results from four, three, and two parasite cultures grown with WT, Δdecr, and Δdecr::DECR L. major, respectively. (B) L. major promastigotes were labeled with ¹³C-labeled linoleic acid and levels of ¹³C label incorporated into citric acid determined by GC/MS. Data represent means with SEM of results from four cultures (*, P < 0.05). (C) Analysis of citric acid mass isotopologues generated after ¹³C linoleic acid labeling. Data represent means with SEM of results from four, three, and two parasite cultures grown with WT, Δdecr, and Δdecr::DECR L. major, respectively (*, P < 0.05).