FIG 9.

SrcA, PmrA, and the canonical UPR protect against calcineurin inhibition. (A) Serial 10-fold dilutions of conidia from the KU70 parental strain and the ΔhacA mutant were incubated for 2 days at 37°C on AMM plates without or with supplementation with Ca²⁺ and in the presence or absence of brefeldin A (BFA), calcofluor white (CFW), or caspofungin (CAS). (B) The indicated strains were incubated for 2 days at 37°C on AMM plates in the presence of cyclosporine (CsA) or FK506. (C) Bright-field and PI-stained fluorescent images of hyphae after 24 h at 37°C in liquid AMM containing CsA or FK506. No fluorescence was observed in untreated controls (see Fig. 8B). Bars: 50 μm. (D) Serial 10-fold dilutions of conidia from mutants lacking srcA and/or pmrA and the KU80 parental strain were spotted onto AMM in the presence or absence of FK506 or CsA and incubated for 2 days at 37°C.