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. 2020 May 27;11:691. doi: 10.3389/fpls.2020.00691

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Copyright © 2020 Zhou, Lin, Lan, Zhang, Liu, Miao, Mou, Nguyen, Wang, Zhang, Zhou, Zhu, Wang, Zhang, Guo, Liu, Jiang and Wan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Seed dormancy phenotypes of OsWRKY29 transgenic plants. (A) OsWRKY29 expression levels in seeds of the NIP, OsWRKY29 Ri and OE lines determined by RT-qPCR. Fresh seeds were collected at 45 days post heading for RT-qPCR analysis. Values are means ± SD (n = 3). The Student’s t-test analysis indicates a significant difference (**P < 0.01). (B) Seed germination percentages of NIP, OsWRKY29 Ri and OE lines after five days of imbibition. Fresh seeds were collected at 45 days post heading for germination test. Values are means ± SD (n = 3), 30 seeds were measured in each replicate. The Student’s t-test analysis indicates a significant difference (*P < 0.05, **P < 0.01). (C) Seed dormancy phenotypes of NIP, OsWRKY29 Ri and OE lines after five days of imbibition. Fresh seeds were collected at 45 days post heading for germination test. Scale bar, 2 cm.