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. 2020 May 27;11:691. doi: 10.3389/fpls.2020.00691

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Copyright © 2020 Zhou, Lin, Lan, Zhang, Liu, Miao, Mou, Nguyen, Wang, Zhang, Zhou, Zhu, Wang, Zhang, Guo, Liu, Jiang and Wan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Subcellular-localization, expression pattern, and transcription activity of the OsWRKY29 gene. (A) Subcellular localizations of the OsWRKY29-GFP fusion protein and GFP-OsWRKY29 fusion protein in rice protoplasts. D53-mCherry was used as the nuclear marker. Scale bar, 10 μm. (B) Relative expression of OsWRKY29 in various tissues including root (2 weeks old), stems (heading stage), leaf (heading stage), leaf sheath (heading stage), panicle (2 cm), Glume (in blossom), and seed (30 DPA). Values are means ± SD (n = 3). (C) Transcription activity assay of OsWRKY29 in rice protoplasts. The reporters and effectors used in the assay were generated as shown. Firefly luciferase (LUC) and renilla luciferase (REN) activities were determined 16 h post-transformation. The relative luciferase activities in control and OsWRKY29-expressed samples were calculated by normalizing the LUC values against REN. Values are means ± SD (n = 3). The Student’s t-test analysis indicates a significant difference (**P < 0.01).