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. 2020 May 27;11:691. doi: 10.3389/fpls.2020.00691

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Copyright © 2020 Zhou, Lin, Lan, Zhang, Liu, Miao, Mou, Nguyen, Wang, Zhang, Zhou, Zhu, Wang, Zhang, Guo, Liu, Jiang and Wan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ABA responses of OsWRKY29 RNA interference (Ri) and overexpression (OE) lines. (A) Seed germination phenotypes of Nipponbare (NIP), OsWRKY29 Ri and OE lines treated with ABA at 7th day. Scale bar, 2 cm. (B–E) Germination time courses in water containing 0 μM ABA (B), 1 μM ABA (C), 3 μM ABA (D), and 5 μM ABA (E). Seed dormancy was broken by drying at 50°C for 72 h. The heat-treated seeds (dormancy released stage) were used for ABA treatment. Values are means ± SD (n = 3).