Figure 4.

Upregulated expression of NF-κB in ARC and co-expression of GRK6 with NF-κB in ARC neurons of CON rats. (a) Compared with CON rats (n = 6), NF-κB protein expression was significantly increased in ARC of NMD rats (n = 5) (**p < 0.01, two-tailed two-sample t-test). (b) The mRNA expression of NF-κB receptor in ARC region of NMD rats was not significantly altered (n = 4, for each group, p > 0.05, two-tailed two-sample t-test). (c and d) Compared with CON rats, NF-κB protein expression was not significantly altered in the BLA and IC regions of NMD rats (n = 4 for each group, p > 0.05, two sample t-test). (e) GRK6-positive cells were colored with green fluorescent secondary antibody. (f) NF-κB-positive cells were colored with red fluorescent secondary antibody. (g) GRK6 and NF-κB was co-expressed in ARC neurons (kelly). Scale bar = 50 µm. (h) Statistical analysis showed that most of GRK6-positive cells and NF-κB-positive cells were co-expressed in ARC neurons (n = 4 rats for each group). GRK6: G protein-coupled kinase 6; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; CON: control; NMD: neonatal maternal deprivation; ARC: arcuate nucleus; BLA: basolateral amygdala; NF-κB: nuclear factor κB; IC: insular cortex.