Table 3. Overview of Ru-BSA Interaction Studies.
| technique | concentrationsa | result | conclusion |
|---|---|---|---|
| PAGE+CuAAC | [Ru] = 75 μM | fluorescent band after light activation + CuAAC | Ru-BSA interaction controlled by light; it withstands CuAAC conditions |
| [BSA] = 15 μM | no fluorescent band in the dark or without click handle | ||
| UV–vis | [Ru] = 15 μM | no change in UV–vis spectrum for activated ruthenium compound in the presence of BSA | Ru-BSA interaction cannot be visualized by UV–vis |
| [BSA] = 15 μM | |||
| ESI-MS | [Ru] = 500 μM | no signal of ruthenated BSA | Ru-BSA interaction too weak for mass spectrometry analysis |
| [BSA] = 100 μM |
a
Ru represents either [1](PF6)2 or [2](PF6)2. Conditions: After a 24-h incubation in the dark (37 °C), the samples were activated by 1 h of green light irradiation at 37 °C and further incubated for at least 24 h.