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. 2020 Jun 3;12:76. doi: 10.1186/s13148-020-00868-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Estimated cell-type proportions and associated variables. a Cell 1 shows both longitudinal and cross-sectional variability. The proportion of cell 1 is negatively correlated with age at visit 2 (r = − 0.48, p value = 0.006, n = 31; black squares and dashed line), but not at visit 1 (r = − 0.13, p value = 0.49, n = 31; red × markers and dotted line), and only slightly at visit 3 (r = − 0.32, p value = 0.10, n = 27; gray circles and solid line). b Cell 3 is associated with cross-sectional variability but no significant longitudinal change. The estimated proportion has a strong positive correlation with age at all three visits. At visit 1, r = 0.36 (p value = 0.05); visit 2, r = 0.57 (p value = 0.0009); visit 3, r = 0.48 (p value = 0.01). c Cell 3 also shows a significantly higher proportion in African-Americans at all three visits (F_2,28 = 15.66, p value < 0.0001 at visit 1). d Cell 2 is also ethnicity-specific and associated with a lower proportion in African-Americans at all three visits (F_2,28 = 4.77, p value < 0.02 at visit 1)