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. 2020 Jun 3;20:512. doi: 10.1186/s12885-020-07002-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Relationships among total PR, PR-A, PR-B and ER and their associations with lymph node status. Scatter plots of total PR vs. PR-A (Panel a), PR-B vs. PR-A (Panel b), and ER vs. PR-A (Panel c). In Panels A-C, the relative gene expression values are represented after Box-Cox transformation. The solid line represents the linear regression line. The correlation coefficients (r) in repeated measures were estimated using linear mixed-effects models. Associations between lymph node status versus total PR (Panel d), PR-A (Panel e), PR-B (Panel f), PR-A/PR-B (Panel g), and ER (Panel h). In Panels D-H, the relative gene expression values are represented after Box-Cox transformation. The solid lines represent the overall mean values. The associations in repeated measures were assessed using a linear mixed-effects model. The transformed expression levels in each group were summarized by mean and standard error of mean (SEM). The fold change (lymph node positive vs. negative) was calculated using the medians of raw expression levels