Fig. 3.

Mifepristone dose sensitivity for inhibition of the transcriptional activity of PR-A compared with PR-B. In Panels a, hormone depleted T47D-A and T47D-B cells were transfected with a plasmid expressing a minimal promoter-luciferase reporter containing a progesterone responsive element (PRE-TATA-LUC) and simultaneously treated with vehicle (DMSO) or 10 nM R5020 (P) plus varying concentrations of RU486 (0.015625 nM- 256 nM) for 48 h. Cells were harvested by preparing lysates for measurement of luciferase activity. In Panel b, T47D-A and T47D-B cells were similarly treated but without transfection. At the end of the treatment, total RNA was isolated and mRNA for the HSD11B2 gene was measured by quantitative RT-PCR. For Panels a and b, error bars have been included to denote SEM for 3 replicates