Table 2.

Characteristics of the primers used for real-time PCR analysis^a

Genes	Primer (from 5’to 3′)	Size, bp	Accession No.
CAT	F: ACATGGTCTGGGACTTCTGG R: TCATGTGCCTGTGTCCATCT	99	XM_021081498.1
Gpx4	F: TGTGGTTTACGGATTCTGG R: CCTTGGGCTGGACTTTCA	181	NM_214407.1
HSP70	F: AGGTGCAGGTGAGCTACAAG R: CTGCGAGTCGTTGAAGTAGG	158	NM_213766.1
SOD2	F: CCTACGTGAACAACCTGAAC R: GATACAGCGGTCAACTTCTC	247	NM_214127.2
TFAM	F: AGATGCTTATAGGGCAGACTGGCA R: ACCTATGTATTGAACTGGCTGGCA	607	NM_001130211.1
MAFbx	F: CCAGAGAGTCGGCAAGT R: GAGGGTAGCATCGCACAAGT	374	NM_001044588
MuRF1	F: AGCACGAAGACGAGAAAATC R: TGCGGTTACTCAGCTCAGTC	150	NM_001184756
GAPDH	F: TCGGAGTGAACGGATTTGGC R: TGACAAGCTTCCCGTTCTCC	189	NM_001206359.1

^aCAT, catalase; Gpx4, glutathione peroxidase 4; HSP70, heat shock protein 70; SOD2, superoxide dismutase 2; TFAM, mitochondrial transcription factor; MAFbx, muscle atrophy F-box; MuRF1, muscular ring finger protein 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase