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. 2020 Jun 3;11:56. doi: 10.1186/s40104-020-00463-0

Table 3.

Characteristics of the antibodies used for western blot analysisa

Antibody Catalog number Source Dilution rate
HSP70 ab5439 Mouse 1:2,000
MAFbx 55,456–1-AP Rabbit 1:600
MuRF1 12,866–1-AP Rabbit 1:500
GAPDH 10,494–1-AP Rabbit 1:7,000
HRP goat anti-rabbit IgG SA00001–2 Goat 1:6,000
HRP goat anti-mouse IgG SA00001–2 Goat 1:5,000

aHSP70, heat shock protein 70; MAFbx, muscle atrophy F-box; MuRF1, muscular ring finger protein 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase