Table 1.
Primer sequences of the qRT-PCR analysis
| Gene | Sequence (5′-3′) | Annealing Temperature | Product (bp) | GeneBank No |
|---|---|---|---|---|
| PCNA | F: GCTCCATCCTGAAGAAGGT | 55 °C | 121 | NM-022381.3 |
| R: TGCACTAAGGAGACGTGAGA | ||||
| Bax | F: GAGACACCTGAGCTGACCTT | 55 °C | 104 | NM-017059.2 |
| R: TCCATGTTGTTGTCCAGTTC | ||||
| Bcl-2 | F: AGTACCTGAACCGGCATCT | 55 °C | 120 | NM-016993.1 |
| R: CCGGTTACTATTCCTGGAGA | ||||
| Caspase-3 | F: CCGGTTACTATTCCTGGAGA | 55 °C | 117 | NM-017008.4 |
| R: TAACACGAGTGAGGATGTGC | ||||
| MCT4 | F: CCTTCCTTCTCACCATCCT | 55 °C | 136 | BC168146.1 |
| R: TCAGTGAAGCCATTGAAGAA | ||||
| MCT2 | F: GGATTGGGATTTGGAAGTAT | 55 °C | 119 | NM-003676.8 |
| R: AGAACTGGACAACACTCCAC | ||||
| GLUT1 | F: TGGCTCCTCATGTCAGAGA | 55 °C | 125 | AJ245935.1 |
| R: AGGATCTCCATGATGCTGTT | ||||
| HK | F: AGAGGCTACGGACAGAGATG | 55 °C | 121 | NM-012734.1 |
| R: AGGAAGTCACCGTGTTCAGT | ||||
| PFK | F: GGCGTGTGTTCATTGTAGAG | 55 °C | 125 | NM-017008.4 |
| R: CTTCAAGTCGTGGATGTTGA | ||||
| PKM | F: ACATCCTGTGGCTGGACTAT | 55 °C | 130 | NM-053297.2 |
| R: TCCACTTCTGTCACCAGGTA | ||||
| LDH | F: GGTTGACAGTGCATACGAAT | 55 °C | 106 | NM-017025.1 |
| R: CCGCCTAAGGTTCTTCATTA | ||||
| GAPDH | F: CCTCAAGATTGTCAGCAATG | 55 °C | 134 | NM-017008.4 |
| R: CAGTCTTCTGAGTGGCAGTG |