Table 3.
DNA primers used for the analysis of efflux transporter and CYP enzyme mRNA expression in the endothelial monolayer from the BBB and BBTB models
| Target | Gene | Primer F/R | Primer sequence |
|---|---|---|---|
| P-gp | ABCB1 | F | CAGACAGCAGCTGACAGTCCAAGAACAGGACT |
| R | GCCTGGCAGCTGGAAGACAAATACACAAAATT | ||
| BCRP | ABCG2 | F | TGGCTGTCATGGCTTGAGTA |
| R | GCCACGTGATTCTTCCACAA | ||
| MRP1 | ABCC1 | F | GTCCTTAAACAAGGAGGACACG |
| R | TCCTTGGAGGAGTACACAACCT | ||
| MRP2 | ABCC2 | F | CCAATCTACTCTCACTTCAGCGAGA |
| R | AGATCCAGCTCAGGTCGGTACC | ||
| CYP1A1 | CYP1A1 | F | TTTTACATCCCCAAGGGGCG |
| R | TCTCACCGATACACTTCCGC | ||
| CYP1B1 | CYP1B1 | F | CCACTGAAGTGGCCTAACCC |
| R | CCACTGAAGTGGCCTAACCC | ||
| CYP2D6 | CYP2D6 | F | TCATCACCAACCTGTCATCGG |
| R | CCTCCGGCTTCACAAAGTGG | ||
| CYP2S1 | CYP2S1 | F | GATCTACAGCCCCTGTTCGG |
| R | TTAATTCCCAAGCCGGACCC | ||
| CYP2U1 | CYP2U1 | F | CAGCCATTTGGGAGAAACCG |
| R | TGTTCTCCCATACACACCCG | ||
| Cyclophilin A | PPIA | F | CTGAGGACTGGAGAGAAAGGAT |
| R | GAAGTCACCACCCTGACACATA |