Table 2.
Comparison of biochemical characterization of recombinant VxAly7D and AlyD
| Parameter | VxAly7D | AlyD |
|---|---|---|
| Origin | V. xiamenensis QY104 | V. splendidus 12B01 |
| Molecular mass | 36.46 kDa | 36.5 kDa |
| Optimal pH | 7.3 | 8 |
| Optimal temperature | 30 °C | 20 °C |
| Optimal NaCl concentration | 100 mM | 400 mM |
| Metal ions | ||
| Inhibition | Cu2+, Ni2+, Zn2+ | Cu2+, Ni2+, Zn2+, Fe2+, Mg2+, Mn2+ |
| Promotion | Ca2+, Fe2+, Mg2+, Mn2+ | Ca2+ |
| EDTA | Inhibit | Inhibit |
| Substrate preference | Sodium alginate (462.40 U/mg) | Alginate (0.5 μM/s/μg) |
| polyG (357.37 U/mg) | polyG (0.41 μM/s μg) | |
| polyM (441.94 U/mg) | polyM (0.25 μM/s μg) | |
| Products | Mono-, di-, trisaccharides | Mono-, di-, pentasaccharides |
| Action mode | Exo-type | Endo-type |
| Reference | In this study | [46] |