Fig. 2.

The role of miR-550a-3-5p in regulation of proliferation, apoptosis, migration, invasion, and EMT. a Quantitative results of cell proliferation in HPV-positive OSCC cells (UPCI:SCC090 and UM-SCC-47) and HPV-negative OSCC cells (Cal-27 and SCC25) with miR-550a-3-5p overexpression (M, mimic-transfected) or inhibition (I, inhibitor-transfected) were presented by comparing to their NC group. b Quantitative results of cell apoptosis in HPV-positive and HPV-negative OSCC cells with miR-550a-3-5p overexpression or inhibition were presented by comparing to their NC group. Images of cell proliferation and flow cytometry were provided in Supplementary Fig. 2 (c) Wound healing assays of HPV-positive and HPV-negative OSCC cells with miR-550a-3-5p overexpression or inhibition. Images were acquired at 0 (white line) and 24 h (blue line) (magnification 200X). Shown were representative images on the left and quantitative analysis of scratch wound closure on the right. d Transwell invasion assays of HPV-positive and HPV-negative OSCC cells with miR-550a-3-5p overexpression or inhibition (magnification 200X). Quantification was shown as proportions of their controls. e mRNA levels of E-cadherin and Vimentin were assessed in Cal-27 and UPCI:SCC090 cells with miR-550a-3-5p overexpression using qRT-PCR. All assays were carried out in triplicate. Results were presented as means ± SD. *P<0.05, **P<0.01, ***P<0.001