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Primers sequences used for SNPs genotyping in the OPG gene
| SNP | Sequence 5′- 3′ | PCR-Product (bp) | Genotyping Method |
|---|---|---|---|
| rs3102734 | Forward: TGCCGGGACGCTATATATAAC | 226 | Restiction digestion (HAEIII) |
| Reverse: TTCTCCCCGCCGGTCCGCT | |||
| rs2073618 | Forward 1: CGGGGACCACAATGAACAAC | 273 | Allele specifc polymerase chain reaction or ASPCR |
| Forward 2: CGGGGACCACAATGAACAAG | |||
| Reverse: CTCTCTCTTGCTGTCTTCCAT | |||
|
rs2228568 rs7844539 |
Forward: TTACAAGGAAACTGGGGAGC | 437 | Sanger Sequencing |
| Reverse: TGTGTGAGGAGGCATTCTTC |
