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Application Protocol
| Liquid period | Liquid | Volume (μL) | VP runs |
|---|---|---|---|
| 1 | Saline | 5 | 3 |
| 2 | Saline | 5 | 3 |
| 3 | Vehicle | 5 | 3 |
| 4 | Vehicle | 5 | 3 |
| 5 | Concentration 1 | 5 | 3 |
| 6 | Concentration 2 | 5 | 3 |
| 7 | Concentration 3 | 5 | 3 |
| 8 | Concentration 4 | 5 | 3 |
| 9 | Concentration 5 | 5 | 3 |
| 10 | Saline | 5 | 3 |
VP, voltage potential.
