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. Author manuscript; available in PMC: 2020 Jun 3.
Published in final edited form as: ACS Chem Biol. 2020 Jan 3;15(1):140–150. doi: 10.1021/acschembio.9b00694

Figure 1.

Figure 1.

Proteomic analysis of HCC4017 cells treated with 35 small molecule chemical probes. (A) Overview of the workflow of the quantitative proteomic experiments. (B) Number of proteins commonly (blue bars) and uniquely (red bars) identified in all TMT sets for HCC4017 cells. (C) Immunoblots of phosphorylated forms of S6, S6K, and ERK in HBEC30KT and HCC4017 cells upon the treatment with the indicated compounds. (D) Percentage of the identified proteins that were found in the COSMIC Census and in the Uniprot/Swiss-Prot database. (E) Quantitative reproducibility of the TMT experiments. (F) TMT results and immunoblots of UBE2C and HELLS levels in HCC4017 cells upon the treatment of the indicated compounds.