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. 2020 Jun 3;39:101. doi: 10.1186/s13046-020-01598-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Circ-STAT3 sponged miR-29a/b/c-3p to up-regulate STAT3. a Influence of circ-STAT3 depletion on STAT3 expression was evaluated by qRT-PCR analysis. Student’s t-test. b RIP assay revealed enrichment of circ-STAT3 and STAT3 pulled down by anti-IgG and anti-Ago2. Student’s t-test. c Venn diagram revealed the number of miRNAs binding both circ-STAT3 and STAT3 based on starBase database.

d The top 10 significantly abnormally expressed miRNAs in HepG2 cells normalized to control cells. e-f qRT-PCR and western blot examined influence of miR-29a/b/c-3p overexpression on expression of STAT3. One-way ANOVA. g Binding sites of wild type/mutant circ-STAT3/STAT3 and miR-29a/b/c-3p. h Luciferase reporter assay revealed luciferase activity of wild and mutant circ-STAT3/STAT3 (3 different mutated circ-STAT3/STAT3 with different mutations for miR-29a/b/c-3p) by miR-29a/b/c-3p mimics. One-way ANOVA. i RNA pull down assay revealed enrichment of miR-29a/b/c-3p pulled down by biotinylated circ-STAT3 and non-biotinylated circ-STAT3. Student’s t-test. j RIP assay revealed enrichment of circ-STAT3, miR-29a/b/c-3p and STAT3 in anti-IgG and anti-Ago2. Student’s t-test. ^**P < 0.01. The symbol “n.s.” indicates no significance.