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. 2020 Jun 1;16:172. doi: 10.1186/s12917-020-02387-3

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Analytical Sensitivity of M. ovipneumoniae real-time RPA (a) and LFS RPA (b) assays. The LOD of the real-time RPA was 1.0 × 10² copies per reaction of M. ovipneumoniae standard DNA, while the LOD of the LFS RPA was 1.0 × 10¹ copies per reaction. Lane 1, 1.0 × 10⁷ copies; lane 2, 1.0 × 10⁶ copies; lane 3, 1.0 × 10⁵ copies; lane 4, 1.0 × 10⁴ copies; lane 5, 1.0 × 10³ copies; lane 6, 1.0 × 10² copies; lane 7, 1.0 × 10¹ copies; lane 8, 1.0 × 10⁰ copies