. 2020 Jun 1;16:172. doi: 10.1186/s12917-020-02387-3

Table 3.

Sequences of the primers and probes for M .ovipneumoniae real-time RPA, LFS RPA and PCR assays

Assay	Primers and probes	Sequence 5′-3′	Amplicon size (bp)	References
real-time RPA	MO-exo-F	TGAGTAACACGTACCTAACCTACCTTTTGGAC	254	This study
	MO-exo-R	TGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTC
	MO-exo-P	TTGGTAGGGTAAAGGCCTACCAAGACGATGA (FAM-dT)(THF)(BHQ1-dT)TTAGCGGGGCCAAGAG-C3-spacer
LFS RPA	MO-nfo-F	TGAGTAACACGTACCTAACCTACCTTTTGGAC	254	This study
	MO-nfo-R	Biotin-TGCTGCCTCCCGTAGGAGTCTGGGCCGTATCTC
	MO-nfo-P	FAM-TTGGTAGGGTAAAGGCCTACCAAGACGATGAT(THF)TTTAGCGGGGCCAAGAG-C3-spacer
real-time PCR	Mo16S_35F	TGGGTGAGTAACACGTACCTAACC	62	[4]
	Mo16S_96R	AGCCGCTGTTTCCAATGG
	Mo16S_60T	FAM-ACCTTTTGGACCGGGATA-MGB
PCR	LMF1	TGAACGGAATATGTTAGCTT	361	[9]
PCR	LMR1	GACTTCATCCTGCACTCTGT