Table 3.
Major characteristics of the included studies
| Study | Study design | Setting (deep dentinal lesion in primary/ permanent teeth) | Setting (human dentine blocks) | Inclusion/exclusion criteria | Test Microbes | Antimicrobial agents | No. of primary/ permanent teeth dentin blocks | Test & control group | Study Period |
|---|---|---|---|---|---|---|---|---|---|
| In vivo studies | |||||||||
| Karched et al., 2019 | In vivo study | ✓ | - | ✓ | Streptococcus mutans |
-38% Silver diamine fluoride -SDF/KI -2%CHX (+ve control) |
25 permanent teeth | - | Evaluation after 48 hours of incubation |
| Milgrom et al., 2018 | RCT- double blind placebo-controlled | ✓ | - | ✓ | Wide array of bacterial taxa (RNA sequencing) | -38% Silver diamine fluoride |
6 primary teeth (2-caries lesion + 1-unaffected surface) (multi species) |
- | Evaluation post SDF 14-21 days |
| Mitwalli et al., 2019 | RCT | ✓ | - | ✓ | Wide array of bacterial taxa through DNA sequencing | -38% Silver diamine fluoride |
20 permanent teeth (multi species) |
- | Evaluation post SDF 4 weeks |
| In vitro studies | |||||||||
| Mei et al., 2013 | In vitro study | - | ✓ | ✓ |
Streptococcus mutans Streptococcus sobrinus Lactobacillus acidophilus Lactobacillus rhamnosus Actinomyces naeslundii |
38% Silver diamine fluoride |
Seventy- two dentin blocks (Biofilm model- mixed culture) |
✓ | Evaluation after 7, 14 and 21 days |
| Chu et al., 2012 | In vitro study | - | ✓ | ✓ |
Streptococcus mutans Actinomyces naeslundii |
38% Silver diamine fluoride |
Thirty-two dentin blocks (Biofilm model- mixed culture) |
✓ | Evaluation after 7 days |
| Mei et al., 2013(a) | In vitro study | - | ✓ | ✓ |
Streptococcus mutans Lactobacillus acidophilus |
38% Silver diamine fluoride |
Thirty dentin blocks (Biofilm model- mixed culture) |
✓ | Evaluation after 7 days |
| Hamama et al., 2015 | In vitro study | - | ✓ | ✓ | Streptococcus mutans |
-Silver diamine fluoride/potassium iodide (SDF/KI) -Chlorhexidine (CHX) + SDF/KI |
45 dentin blocks (Biofilm model-single species) |
X | Evaluation after 5 minutes of dentine disc treatment |
| Besinis et al., 2014 | In vitro study | - | ✓ | ✓ | Streptococcus mutans |
-Nanosilver (AgNPs) -Silver nitrate (AgNO3) -Chlorhexidine |
48, coronal dentin discs (Biofilm model-single species) |
✓ | Evaluation after 24-hours |
✓=mentioned/present, in the included publication; X =not mentioned, in the included publication