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. 2020 Jun 1;20:30. doi: 10.1186/s12896-020-00623-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — CD19 is expressed on the surface of Caco-2 cells and do not hinder their ability to form cysts. a BLI killing assay of Caco-2 cells expressing CD19 or not, co-cultured with CD19CAR or Mock T cells (E:T ratio of 1:10). Data represent mean ± S.D. of hexaplicates. Representative data from one of three experiments are shown. Statistics analysis were conducted from timepoints 3 to 7 (2-way ANOVA). b Time lapse of Caco-2 GFP+/CD19+ cysts formation observed with a high-throughput microscope. Scale bar represents 20 μm. c Airyscan micrographs showing the establishment of a basal/apical polarity on a Caco-2 organoid marked by Hoechst 33342, PKC-ζ, E-cadherin and actin. Scale bar represents 10 μm