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. 2020 Jun 3;11:215. doi: 10.1186/s13287-020-01732-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — LIPUS and 4-PBA decreased alveolar bone absorption in experimental periodontitis in rats. a 4-step surgery procedures including separating the connective gingiva, ringing the ligature wire of the first maxillary molar, fastening the ligature, and ensuring the tight touch to the teeth to induce the experimental periodontitis in rats. b Schematic of 2 phases of the experimental periodontitis induction and treatment. c Picture of the LIPUS treatment in rat’s oral. The blue arrow is the ultrasonic probe of the LIPUS. d, f The alveolar bone absorption of SD rats (n = 3) was determined by micro-CT (d) and H&E staining (f). e The quantity results of the micro-CT, the absorption of the alveolar bone ratio (ABLs/RBLs) variable is used. The results of micro-CT and H&E staining showed less alveolar bone loss in both the LIPUS and 4-PBA treatment groups compared with the periodontitis group. Guide line CEJ means the parallel line of cemento-enamel junction (CEJ), and guide line ABC means the parallel line of alveolar bone Climax. The representative results were from three independent experiments. The error bars represent the S.D. from the mean values. *p < 0 .05; **p < 0 .01; ***p < 0 .001; ****p < 0 .0001