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. 2020 Jun 3;11:210. doi: 10.1186/s13287-020-01714-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Expression changes of integrin αV, α2, and α3 during osteogenic differentiation of hPDLCs. hPDLCs were incubated for 7 days with BMP-2, followed by treatment with ODM for additional 7 days for mineralization. a Osteogenic differentiation of hPDLCs. Osteogenic and odontogenic markers (BSP, DMP-1, OC, OP, Runx2) and ligamentogenic marker (SCX) were analyzed by quantitative PCR. b, c Expression changes of integrins during osteogenic differentiation of hPDLCs. Cell surface expression of integrins in osteogenic hPDLCs was analyzed by flow cytometry. Values are depicted as MFIs at the indicated culture conditions. Black, incubation in media without BMP-2 treatment; blue, incubation in media with BMP-2 treatment; green, incubation in ODMs with BMP-2. *, p < 0.05; **, p < 0.01; ***, p < 0.005; ns, not significant