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. 2020 Jun 3;11:210. doi: 10.1186/s13287-020-01714-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Expression changes of integrin αV during osteogenic differentiation of hMSCs after cell sorting. a Flow cytometric analysis of integrin αV-high and αV-low hMSCs after MR1-B1-based magnetic cell sorting. hMSCs were cultured in ODM for 21 days after MR1-B1-based magnetic cell sorting. To inhibit TGF-β1 signaling that originated from the culture medium, SB431542 was added to ODM after 7 days of osteogenic differentiation. The cells were detached and analyzed at days 14 and 21 by flow cytometry. b Statistic analysis of a. Values are depicted as relative MFIs of sorted hMSCs at the indicated days compared to sorted hMSCs cultured in normal medium. **, p < 0.01; ***, p < 0.005. c Proposed model of expression dynamics of integrin α2, α3, and αV during osteogenic differentiation of hMSCs. Upon osteogenic differentiation of hMSCs, integrin α2 expression is rapidly downregulated and integrin α3 and αV expression is gradually upregulated upon osteogenic differentiation of hMSCs. Integrin αV expression is more dramatically induced even in integrin αV-low hMSCs, suggesting that integrin αV induction is needed for OB differentiation of hMSCs