Figure 2.

APC/C^TE Mediates the Opposite Effects of ABA and GA on Root Growth and Tillering.

(A) to (D) Seedling height and total root length of 3-y–old wild-type (WT), te, and OE17 seedlings treated with different doses of GA₃ or ABA for 10 d. CK, no phytohormone treatment. Values are means ±sd of 15 seedlings from one of three independent experiments with similar results. The letters a, b, c, d, e, and f indicate significant differences at P < 0.01, and ab at P < 0.05 according to two-way ANOVA test with Tukey correction.

(E) The upper representation shows the phenotype of tissue cultured OsABA1 knockout plant. The lower representation shows the deletion of OsABA1 using CRISPR/CAS9 technology. Shading and colored bases show the single guide DNA site. Scale bar, 2 cm.

(F) and (G) The tiller number (F) and tiller phenotypes (G) of 3-month–old wild-type, te, and OE17 plants treated with 0.05 μM of ABA or 0.05 μM of GA₃ for 1.5-month. Values are means ±sd of 15 seedlings from one of three independent experiments with similar results and *P < 0.05, Student’s t test (two-tailed) in (F). Scale bar, 2 cm; arrowheads denote the tillers in (G).