Figure 5.

Subcellular Localization of Arabidopsis TPS1.

The tps1-1 mutant was complemented with various constructs encoding GFP-tagged forms of the TPS1 protein, and the localization of the GFP fusion proteins was examined by laser scanning confocal microscopy in guard cells on the abaxial surface of the leaf (A), roots of 8-d old seedlings (B), and SAMs (C). A complemented line expressing the native (i.e., nontagged) TPS1 protein (TPS1; top row) was used as a negative control for autofluorescence. The constructs used for complementation of tps1-1 are shown in Figure 1. The green and red channels show GFP and chlorophyll autofluorescence, respectively, and the merged images show the GFP signal superimposed on the bright-field images. In (C), the dotted white lines mark the outer surface of the meristem and leaf primordia. Bars = 30 μm.