. 2020 May 27;11:529. doi: 10.3389/fphys.2020.00529

TABLE 2.

Effect of Wooden Breast on density, relative density, and heterogeneity of Pectoralis major muscle satellite cell populations in broiler chickens at 43 days of age.

Cell population^2,3	Wooden Breast Score¹			SEM⁴	P-value
	Normal (0)	Mild (1)	Severe (2)
Density, cells per mm²
Myf-5-:MyoD−:Pax7− (non-myogenic)	267.43	308.41	403.76	56.25	0.236
Myf-5+	123.62^x	187.12^y	121.38^x	22.83	0.092
MyoD+	3.75^a	30.63^b	9.00^a	7.06	0.032
Pax7+	13.50^x	26.25^xy	52.87^y	11.43	0.067
Myf-5+:MyoD+	58.13^a	177.13^b	57.37^a	36.38	0.046
Myf-5+:Pax7+	0.75	7.50	3.75	2.79	0.252
MyoD+:Pax7+	10.50	12.00	3.00	4.08	0.269
Myf-5+:MyoD+:Pax7+	30.00^a	72.37^b	41.87^ab	11.63	0.048
Relative density, % of total DAPI+
Myf-5−:MyoD−:Pax7− (non-myogenic)	52.71^ab	37.57^a	58.29^b	0.06	0.050
Myf-5+	24.37	22.80	17.52	0.04	0.272
MyoD+	0.74^a	3.73^b	1.29^a	0.01	0.033
Pax7+	2.67^a	3.19^a	7.63^b	0.01	0.042
Myf-5+:MyoD+	11.46	21.58	8.28	0.05	0.115
Myf-5+:Pax7+	0.15	0.92	0.54	0.01	0.452
MyoD+:Pax7+	2.07	1.46	0.43	0.01	0.121
Myf-5+:MyoD+:Pax7+	5.91	8.82	6.05	0.01	0.243
Relative density, % of total myogenic
Myf-5+	51.54	36.51	42.02	0.06	0.174
MyoD+	1.56	5.98	3.12	0.01	0.105
Pax7+	5.63^ab	5.12^a	18.30^b	0.03	0.029
Myf-5+:MyoD+	24.23	34.56	19.86	0.06	0.191
Myf-5+:Pax7+	0.31	1.46	1.29	0.01	0.702
MyoD+:Pax7+	4.38^a	2.34^ab	1.04^b	0.01	0.085
Myf-5+:MyoD+:Pax7+	12.51	14.12	14.50	0.03	0.864

¹The Pectoralis major muscles of all birds were visually assessed and scored on a 3-point scale (0 = normal; 1 = mild; 2 = severe) for Wooden Breast (n = 8 per score). ²DAPI = 4′6-diamindino-phenylindole nuclear counterstain. All subsequent cell populations may be assumed to be DAPI+. ³Total describes every cell positive for the specific immunofluorescence target protein, regardless of the status of other targets; Non-myogenic describes cells not positive for Myf-5, Pax7, MyoD, or any combination thereof; Myogenic describes cells positive for Myf-5, Pax7, MyoD, or any combination thereof. ⁴SEM = highest standard error of the pair-wise mean comparisons. ^a,bMeans within the same row with different superscripts differ P ≤ 0.05. ^x,zMeans within the same row with different superscripts differ 0.0501 ≤ P ≤ 0.10.