Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2021 Jan 1.

Published in final edited form as: J Orthop Res. 2019 Sep 6;38(1):139–149. doi: 10.1002/jor.24451

Figure 5. — Flexor explants were incubated with CM with or without DEX from day 2–7 to simulate BTM injury and treated injury in the absence of living muscle and bone (n=5/group/timepoint). (A) Otherwise healthy FDL explants () remain viable for the entire 7-day culture period. (B) FDL+CM explants have reduced viability after 24 hours in CM that persists through day 7. (C) FDL+CM+DEX explants demonstrated rescued viability compared to the FDL+CM group. While there were no differences in (D) metabolism, (E) DNA, (F) total collagen, (G) sGAG, (H) sGAG synthesis, and (I) proliferation were all reduced in FDL+CM explants (). DEX treatment () further reduced metabolic activity, sGAG synthesis, and proliferation but rescued matrix loss in total collagen and sGAG. Data are presented as mean ± 95% confidence interval where significant differences compared to the FDL control group are represented by stars (*) and compared to the FDL+CM group are represented by the scarab symbol (¤). Trends are additionally denoted by a hashtag symbol (#).

Inline graphic — Flexor explants were incubated with CM with or without DEX from day 2–7 to simulate BTM injury and treated injury in the absence of living muscle and bone (n=5/group/timepoint). (A) Otherwise healthy FDL explants () remain viable for the entire 7-day culture period. (B) FDL+CM explants have reduced viability after 24 hours in CM that persists through day 7. (C) FDL+CM+DEX explants demonstrated rescued viability compared to the FDL+CM group. While there were no differences in (D) metabolism, (E) DNA, (F) total collagen, (G) sGAG, (H) sGAG synthesis, and (I) proliferation were all reduced in FDL+CM explants (). DEX treatment () further reduced metabolic activity, sGAG synthesis, and proliferation but rescued matrix loss in total collagen and sGAG. Data are presented as mean ± 95% confidence interval where significant differences compared to the FDL control group are represented by stars (*) and compared to the FDL+CM group are represented by the scarab symbol (¤). Trends are additionally denoted by a hashtag symbol (#).