Figure 4.

Transcript expression levels of 3 anti-viral biomarkers [viperin-b (A), mx-b (B), and isg15-a (C)] in the head kidney of salmon held at 12°C (under normoxia) or 20°C (under normoxia or mild hypoxia) (i.e., treatment) 6, 12, 24, and 48 h post-intraperitoneal injection with PBS or Forte V II (i.e., injection group). Different capital letters indicate a significant difference (P < 0.05) among treatments within an injection group at a given time point (e.g., the three treatments 6 HPI after PBS injection) whereas dissimilar lower case letters indicate a significant difference over time within a treatment/group combination (e.g., control PBS-treated at 6, 12, 24, and 48 HPI). An asterisk (*) indicates a significant difference between PBS and Forte V II injection groups within a treatment at the same time point post-injection. The arrows indicate a significant difference compared with initial (constitutive expression) levels, within an injection group. Transcript levels are presented as mean ± SE relative quantity (RQ) values (i.e., values for the transcript of interest were normalized to both etif3d and pabpc1 transcript levels and were calibrated to the individual with the lowest normalized expression level of that given transcript). N = 7–8.