Table 1.
Primers used in the Real-time quantitative polymerase chain reaction (qPCR) analyses.
| Gene of interest | Nucleotide sequence (5′-3′) | bEfficiency (%) | Amplicon size (bp) | (GenBank Acc. No.) | References |
|---|---|---|---|---|---|
| Interleukin 1 beta (il1b) | F: GTATCCCATCACCCCATCAC R: TTGAGCAGGTCCTTGTCCTT |
99 | 119 | AY617117 | (47) |
| Interleukin 8a (cxcl8, alias il8-a) | F: GAAAGCAGACGAATTGGTAGAC R: GCTGTTGCTCAGAGTTGCAAT |
104 | 99 | BT046706 | (47) |
| Cyclooxygenase-2 (cox2) | F: ACCTTTGTGCGAAACGCTAT R: GAGTAGGCCTCCCAGCTCTT |
109 | 113 | AY848944 | (48) |
| Cathelicidin b (camp-b) | F: AGACTGGCAACACCCTCAAC R: TTGCCTCTTCTTGTCCGAAT |
103 | 112 | AY360357 | (49) |
| Hepcidin a (hamp-a) | F: ATGAATCTGCCGATGCATTTC R: AATGGCTTTAGTGCTGGCAG |
96 | 134 | BT125319 | (49) |
| Toll-like receptor 5a (soluble) (stlr5-a) | F: ATCGCCCTGCAGATTTTATG R: GAGCCCTCAGCGAGTTAAAG |
96 | 103 | AY628755 | (50) |
| Interferon gamma (ifng) | F: CCGTACACCGATTGAGGACT R: GCGGCATTACTCCATCCTAA |
103 | 133 | AJ841811 | (48) |
| Interferon regulatory factor 7b (irf7-b) | F: GTCAGTGGTAAAATCAACACGC R: CACCATCATGAAACGCTTGGT |
91 | 99 | FJ517644 | (48) |
| Signal transducer and activator of transcription 1b (stat1-b) | F: GTTCAGGATGCAGAGCATGA R: CCATCCCATTCACCTCTTGT |
99 | 109 | BT048927 | (48) |
| Radical S-adenosyl methionine domain containing protein 2b (rsad2-b, alias viperin-b) | F: TTCCTGGCATGGATAGGTGT R: CTTGGAGTTGTCGCTGGTTT |
102 | 113 | DY728694 | Caballero-Solares et al. (in preparation) |
| Interferon-induced GTP-binding protein b (mx-b) | F: ACGCACCACTCTGGAGAAAT R: CTTCCATTTCCCGAACTCTG |
94 | 184 | BT044881 | (48) |
| Interferon stimulated gene 15a (isg15-a) | F: AAAGTGGCCACAACAAAGCAG R: ATAGGAGCGGGCTCCGTAATC |
90 | 140 | BT049918 | (48) |
| aEukaryotic translation initiation factor 3 subunit D (etif3d) | F: CTCCTCCTCCTCGTCCTCTT R: GACCCCAACAAGCAAGTGAT |
94 | 105 | GE777139 | (48) |
| aPolyadenylate-binding protein 1 (pabpc1) | F: TGACCGTCTCGGGTTTTTAG R: CCAAGGTGGATGAAGCTGTT |
102 | 108 | EG908498 | (48) |
a
Normalizers.
b
Amplification efficiencies were calculated using a 5-point 1:3 dilution series starting with cDNA representing 10 ng of input total RNA. See methods for details.