| pZL1939 |
pRS416-PUF3-HA, expressing PUF3 with a C-terminal 3xHA tag under the control of the endogenous promoter. |
This study |
Figure 1, Figure 2C, Figure 3, Figure 4A, and Figure S1 |
| pZL3340 |
pRS416-TEF2p-PUF3-HA, expressing PUF3 with a C-terminal 3xHA tag under the control of strong TEF2 promoter. |
This study |
Figure 2, A and B |
| pZL3338 |
pRS415-HRR25-myc, expressing HRR25 from the endogenous promoter with a 3xMyc epitope tag at the C-terminus. |
Collins et al. (2017) |
Figure 4A and Figure S1 |
| pZL3542 |
pRS424-ADH1-GBD. |
Collins et al. (2017) |
Figure 4, B and C |
| pZL3557 |
pRS424-ADH1-GBD-HRR25. HRR25 ORF was cloned into the BamHI and SalI sites of pZL3542. |
Collins et al. (2017) |
Figure 4, B and C |
| pZL3740 |
pRS424-ADH1-GBD-HRR25(K38A). The kinase-dead mutant allele of HRR25, hrr25(K38A), was cloned into pZL3542. |
This study |
Figure 4B |
| pZL3736 |
pRS424-ADH1-GBD-HRR25(KD). The DNA sequence encoding the Hrr25 kinase domain was cloned into pZL3542. |
This study |
Figure 4C |
| pZL3744 |
pRS424-ADH1-GBD-HRR25ΔC. The DNA sequence encoding a C-terminal truncation construct of Hrr25 was cloned into pZL3542. |
This study |
Figure 4C |
| pZL3742 |
pRS424-ADH1-GBD-HRR25ΔM. The DNA sequence encoding a middle region truncation construct of Hrr25 was cloned into pZL3542. |
This study |
Figure 4C |
| pMB218 |
pRS424-ADH1-GBD-HRR25Μ. The DNA sequence encoding the middle region of Hrr25 was cloned into pZL3542. |
This study |
Figure S2 |
| pMB220 |
pRS424-ADH1-GBD-HRR25C. The DNA sequence encoding the C-terminal region of Hrr25 was cloned into pZL3542. |
This study |
Figure S2 |
| pZL3805 |
pRS424-ADH1-GBD-HRR25(M+C). The DNA sequence encoding the middle region and the C-terminal domain of Hrr25 was cloned into pZL3542. |
This study |
Figure S2 |
| pZL3539 |
pRS415-TEF-GAD. |
This study |
Figure 4, B and C, and Figure S2 |
| pMB212 |
pRS415-TEF-GAD-PUF3. The PUF3 ORF was cloned into the BamHI and XhoI sites of pZL3539. |
This study |
Figure 4B and Figure S2 |
| pMB214 |
pRS415-TEF-GAD-PUF3N. A DNA sequence encoding the N-terminal domain (amino acid residues 1–533) of Puf3 was cloned into pZL3539. |
This study |
Figure 4, B and C, and Figure S2 |
| pMB216 |
pRS415-TEF-GAD-PUF3C. A DNA sequence encoding the C-terminal domain (amino acid residues 511–879) of Puf3 was cloned into pZL3539. |
This study |
Figure 4, B and C, and Figure S2 |
| pAB101 |
pET21a-HRR25ΔC, expressing a C-terminal truncation construct of Hrr25 with a C-terminal 6xHis tag. |
This study |
Figure 5, A and B |
| pAB104 |
pET21a-HRR25(K38A)ΔC, expressing a C-terminal truncation construct of Hrr25(K38A) with a C-terminal 6xHis tag. |
This study |
Figure 5A |
| pZL3359 |
pET24a-PUF3-HA, expressing Puf3 with a C-terminal 3xHA tag and a 6xHis tag. |
This study |
Figure 5, A and B |
| pZL3419 |
pET24a-PUF3N-HA, expressing the N-terminal domain of Puf3 with a C-terminal 3xHA tag and a 6xHis tag. |
This study |
Figure 5, A and B |
| pZL3421 |
pET24a-PUF3C-HA, expressing the C-terminal domain of Puf3 with a C-terminal 3xHA tag and a 6xHis tag. |
This study |
Figure 5, A and B |
| pZL1760 |
pET24a-MKS1, expressing Mks1 with a C-terminal His6 tag in E. coli. |
Liu et al. (2003) |
Figure 5C |
| pZL971 |
pET24a-RTG2, expressing Rtg2 with a C-terminal His6 tag in E. coli. The RTG2 ORF was cloned into the SacI and XhoI sites of pET24a. |
This study |
Figure 5C |
| pZL4262 |
pRS416-PUF3-HA, expressing PUF3 with a C-terminal 3xHA tag. The genomic DNA of CEN PAK background strain YL27 (wild-type PUF3) was used to amplify the PUF3 DNA fragment for cloning into a pRS416-HA plasmid. |
This study |
Figure 5D |
| pZL4264 |
pRS416-puf3 (24A)-HA, expressing puf3(24A) with a C-terminal 3xHA tag. The genomic DNA of the CEN PAK background strain YL27 (wild-type PUF3) was used to amplify the PUF3 DNA fragment for cloning into a pRS416-HA plasmid. |
This study |
Figure 5D |
| pZL4257 |
pET24a-PUF3-HA, expressing Puf3 with a C-terminal 3xHA tag and a 6xHis tag. The PUF3 coding sequence was amplified using yeast YL28 genomic DNA and cloned into pET24a. |
This study |
Figure 5E |
| pZL4258 |
pET24a-puf3 (24A)-HA, expressing a Puf3(24A) mutant with a C-terminal 3xHA tag and a 6xHis tag. The puf3(24A) coding sequence was amplified using yeast YL632 genomic DNA and cloned into pET24a. |
This study |
Figure 5E |
| pZL3507 |
pRS416-ADH1-GFP-CYC1ter, expressing GFP under the control of ADH1 promoter and the 3′-UTR of CYC1. |
This study |
Figure 6 |
| pZL3407 |
pRS416-ADH1-GFP-CYT2ter |
This study |
Figure 6 |
| pZL3510 |
pRS416-ADH1-GFP-CYT2ter (Puf3 site mutant) |
This study |
Figure 6 |
| pZL3416 |
pRS416-ADH1-GFP-PET123ter |
This study |
Figure 6 |
| pZL3512 |
pRS416-ADH1-GFP-PET123ter (Puf3 site 1 mutant) |
This study |
Figure 6 |
| pZL3519 |
pRS416-ADH1-GFP-PET123ter (Puf3 site 1,2,3 triple mutant) |
This study |
Figure 6 |
| pZL4248 |
pRS416-MKS1-GFP-CYC1ter, expressing GFP under the control of the MKS1 promoter and the 3′-UTR of CYC1. |
This study |
Figure S3 |
| pZL4250 |
pRS416-MKS1-GFP-CYT2ter |
This study |
Figure S3 |
| pZL4252 |
pRS416-MKS1-GFP-CYT2ter (Puf3 site mutant) |
This study |
Figure S3 |
| pZL4253 |
pRS416-MKS1-GFP-PET123ter |
This study |
Figure S3 |
| pZL4255 |
pRS416-MKS1-GFP-PET123ter (Puf3 site 1 mutant) |
This study |
Figure S3 |
| pZL4256 |
pRS416-MKS1-GFP-PET123ter (Puf3 site 1,2,3 triple mutant) |
This study |
Figure S3 |
