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. 2020 Mar 30;215(2):483–495. doi: 10.1534/genetics.120.303125

Figure 6.

Figure 6

ELT-3 is a regulator of collagen gene expression. (A) elt-3 was knocked down in wild-type (N2) animals and messenger RNA expression of indicated collagens and collagen regulators was measured using nCounter assay. Expression is shown as fold change relative to treatment with empty vector control (red line). Each dot indicates a single replicate, bars represent the mean, and error bars are SD. * P <0.05 (t-test). (B) Comparison of mean expression values for indicated treatments. L1 arrest and C. aquatica exposure are relative to animals grown on E. coli OP50. elt-3 RNAi is shown relative to empty vector control in E. coli HT115. (C) Analysis of rolling in ERT60 rol-6(su1006)T and elt-3(gk121); rol-6(su1006)T animals under well-fed conditions. (D) Rolling in response to indicated exposures in elt-3(gk121); rol-6(su1006)T in conditions that generated higher penetrance of rolling (populations that were routinely prepared from egg preps and allowed to starve before collecting eggs produced increased numbers of rollers). Statistical analysis by one-way ANOVA with Dunnett’s multiple comparison test. Asterisks indicate P-values (* P <0.05, ** P <0.005, *** P <0.0005).