Figure 3.

Appearance of misoriented microtubules in nudE mutant axons does not depend on microtubule nucleation machinery. (A and B) Eliminating either dGrip75 (A) or cnn (B) does not affect the microtubule polarity phenotype of nudE^39A/Df mutant axons. n = 23 (control), 22 (dGrip75^175/Df), 34 (nudE^39A/Df), and 33 (dGrip75^175/Df; nudE^39A/Df) axons (A) and n = 16 (control), 16 (cnn^HK21/Df), 18 (nudE^39A/Df), and 24 (cnn^HK21/Df; nudE^39A/Df) axons (B) in replicates as indicated. **P = 0.01–0.001 ***P = 0.001–0.0001, and ****P<0.0001; Kruskal–Wallis test with post hoc Dunn’s multiple comparison analysis. ANOVA with Tukey’s post-hoc anaylsis. All data are mean ± SD. cnn, centrosomin; n.s., not significant.