Figure 2.

Methylation of the nonautonomous Mu1 element in the presence and absence of an active MuDR element. (A) Percent methylated cytosines in sequences flanking the Mu1 insertion, as well as the 5′ and 3′ portions of the Mu1 TIR. Cytosines are classified by sequence context, with “H” meaning any nucleotide except guanine. (B) A graphic depiction of the same patterns of cytosine methylation. For this and all subsequent depictions of cytosine methylation, filled circles indicate methylated cytosines in the CG (red), CHG (blue), and CHH (green) sequence contexts. Open circles represent unmethylated cytosines. Each line represents one clone from a PCR amplification of a bisulfite-treated DNA sample.