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. 2020 May 14;42(1):463–473. doi: 10.1080/0886022X.2020.1762647

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — DACH1 was a direct target of miR-218. (a) The website Targetscan was used to predict the binding sites between miR-218 and the 3′-UTR region of DACH1. (b) A luciferase reporter assay was used to determine the luciferase activities in different groups. (c) Expression of DACH1 in tissues was analyzed by bioinformatics tools in GSE30528 dataset. (d) Expression of DACH1 under HG conditions. QRT-PCR (e, f) and western blot (g, h) were adopted to detect the mRNA and protein levels of DACH1 in different groups in HEK-2 cells. **p < 0.01. NC: negative control; WT: wide type; MUT: mutant type; DKD: diabetic kidney disease; HG: high glucose; Con: control.