KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
NEB DH5α competent E.coli	NEB	cat#C2987
BL21(DE3) competent E.coli	NEB	cat#C2527
Chemicals, Peptides, and Recombinant Proteins
m7GpppG	NEB	cat#S1405S
Sypro Orange q	Thermo Fischer Scientific	cat#S6650
T4 PNK	NEB	cat#M0201
vaccinia capping enzyme	NEB	cat#M2080
α-32P-GTP	Perkin Elmer	cat#BLU506H250UC
His-Pur NiNTA	Thermo Fischer Scientific	cat#88222
HiTrap Q	GE	cat#17115401
HiTrap SP	GE	cat#17115201
HiTrap Butyl	GE	cat#28411005
10K MWCO	Millipore	cat#C7715
calf intestinal phosphatase	Roche	cat#11097075001
Deposited Data
Apo X-ray crystal structure of DM15	(Lahr et al., 2015)	PDBID: 4ZC4
F844YR847E DM15 X-ray crystal structure	This study	PDBID: 6PW3
Oligonucleotides
Forward and reverse primers for K924D Mutation of LARP1 DM15 5’CTGGGCCTTCTTGAAA TATTCCGACGCCAAAAAT TTGGAC3’ 5’GTCCAAATTTTTGGCGTC GGAATATTTCAAGAAGGC CCAG3’	Sigma	N/A
Forward and reverse primers for K924A Mutation of LARP1 DM15 5’CTGGGCCTTCTTGAAATATTCCGCAGCCAAAAAT TTGGAC3’ 5’GTCCAAATTTTTGGCTGC GGAATATTTCAAGAAGGC CCAG3’	Sigma	N/A
Forward and reverse primers for K921A Mutation of LARP1 DM15 5’caaatttttggctttggaatatgccaagaaggcccagaacttctcc 3’ 5’ggagaagttctgggccttcttggcatattccaaagccaaaaatttg3’	Sigma	N/A
Forward and reverse primers for K815A Mutation of LARP1 DM15 5’ cacaacacgtctaccatgcgtatcgtaggcgctgcc 3’ 5’ ggcagcgcctacgatacgcatggtagacgtgttgtg 3’	Sigma	N/A
Forward and reverse primers for F844YR847E Mutation of LARP1 DM15 R847E 5’ catctttttgttgaagtgatcctcgaggaagaaggaccagaagcg 3’ 5’ cgcttctggtccttcttcctcgaggatcacttcaacaaaaagatg 3’ F844YR847E 5’catacatctttttgttgaagtgatcctcgaggaaataggaccagaagcggaagagtgtgt 3’ 5’acacactcttccgcttctggtcctatttcctcgaggatcacttcaacaaaaagatgtatg 3’	Sigma	N/A
Splint adapter 5’ CTTGAAGCAGCTGAACGCCTCCGAGGCGCCACGGAAAAGAGG 3’	Sigma	N/A
Recombinant DNA
human LARP1 isoform 2	ThermoFisher	BC033856
Software and Algorithms
PDB2PQR 2.1.1	(Dolinsky et al., 2007; Dolinsky et al., 2004)	http://nbcr-222.ucsd.edu/pdb2pqr_2.1.1/
Ambertools18	(Case et al., 2017)	http://ambermd.org/AmberTools.php
NAMD 2.9	(Kale et al., 1999; Phillips et al., 2005)	http://www.ks.uiuc.edu/Research/namd/
MDAnalysis 0.16.2	(Michaud-Agrawal et al., 2011)	https://www.mdanalysis.org/
FTMap	(Kozakov et al., 2015; Ngan et al., 2012)	https://ftmap.bu.edu/
VMD	(Humphrey et al., 1996)	https://www.ks.uiuc.edu/Research/vmd/
Phenix 1.13–2998	(Adams et al., 2010)	https://www.phenix-online.org/
POVME 2.0	(Durrant et al., 2014)	rocce-vm0.ucsd.edu/data/sw/hosted/POVME/
QuantStudio™ Design & Analysis Software V 8.1.0.0	ThermoFisher Scientific	https://www.thermofisher.com/us/en/home/technical-resources/software-downloads/ab-quantstudio-3-and-5-real-time-pcr-system.html
PyMOL Molecular Graphics System, Version 1.8.6.0.	(DeLano, 2002)	https://pymol.org/2/
GraphPad Prism 7.0	GraphPad Software	Prism - graphpad.com
SPSS Statistics V.25	IBM	https://www.ibm.com/products/spss-statistics