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. 2020 May 13;27(1):691–702. doi: 10.1080/10717544.2020.1754528

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Evaluation of anti-tumor effect of iVR1-NP-Apa/Sal in vitro. (A) Cell growth rate of the MKN45/MDR cells after various treatments determined by evaluation of the absorbance values using the CCK-8 method. (B) Quantitative analysis of the cell apoptosis rate after treated with different nanoparticle formulations by the Annexin v-FITC/PI double staining method. (C) Qualitative evaluation of the cell apoptosis rate after various treatments through the Annexin v-FITC/PI double staining approach. (D) Cell migration rate and invasion rate were, respectively, investigated by the wound healing assay (the figures above) and trans-well experiment (the figures below). Quantitative analysis the migration rate (E) and invasion rate (F) of MKN-45/MDR cells after treated by different strategies. *p < 0.05 signally different from the group of Free Apa. #p < 0.05 signally different from the group of NPs-Apa/Sal.