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High phosphate induced osteoblast differentiation and calcification in VSMCs. (A) Alizarin red staining in general; (B) Alizarin red staining under microscope; (C) Compared the expression of Bone Morphogenetic Protein 2 (BMP2), Run-related transcription factor 2(RUNX2) and osteocalcin in RT-qPCR; (D) Compared the intracellular calcium concentration of the two groups.
