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HBsAg structure stability in presence of C-terminus mutations associated with HBsAg <1,000IU/ml. The histogram shows the variation of HBsAg C-terminus stability in presence of mutations (single or in pairs) associated with lower HBsAg levels. HBsAg C-terminus stability was measured by calculating ΔΔG by STRUM (Quan et al., Bioinformatics, 2016). ΔΔG [wt-mutated] < 0 indicates a reduced stability in presence of the mutations.
