Figure 1.

SC phenotypes in the sciatic nerve of ALS patients. GFAP and S100β immunofluorescence confocal characterization of SCs in 10 μm sections of the sciatic nerve and one ventral root of ALS patients as compared with control donors. (a–b) 3 sciatic nerves from control donors were analyzed in longitudinal sections (upper panels) and cross-sections (lower panels). Note low GFAP expression, while S100β was restricted to Schmidt-Lanterman clefts (yellow arrowheads in inset). (c–d) 4 sciatic nerves from ALS patients were analyzed in longitudinal and cross-sections. Note GFAP and S100β increase in denervated and myelinating SCs, respectively, labeling different subsets of cells. (e) In comparison, GFAP and S100β were also expressed in different subsets of SCs in the ventral root from one ALS patient. Scale bars: 100 μm in and (c), 10 μm in (b), (d), and (e). 334×168mm (300 × 300 DPI)