FIGURE 3.

Immunohistochemical staining of CXCR4 and CXCR7 in prostatic urethral tissues from LFD- and HFD-fed mice. Representative bright field images of prostatic urethral tissues stained for CXCR4 (left) or CXCR7 (right) protein expression from LFD- or HFD-fed mice. Photomicrographs show tissues stained with anti-rat secondary antibody [indicated as Low Fat Diet or High Fat Diet], with both rat anti-mouse primary CXCR4 and anti-rat secondary antibodies [indicated as Low Fat Diet CXCR4 or High Fat Diet CXCR4] fed regular drinking water (indicated as +CXCR4 Antagonist [−]), or fed drinking water containing CXCR4 Antagonist III (indicated as +CXCR4 Antagonist CXCR4). Similar nomenclature is used for the right panels except that the antibodies used were goat anti-rabbit secondary antibody or rabbit anti-mouse primary CXCR7 antibody. Human breast cancer and mouse brain were stained with secondary alone or primary + secondary antibodies as indicated. Tissues stained with primary CXCR4 or CXCR7 antibodies demonstrate expression of both receptors in both glandular and stromal areas. Treatment with the CXCR4 Antagonist did not increase or diminish expression of CXCR4 or CXCR7. U, urethra. Scale bar = 100 μm. [Color figure can be viewed at wileyonlinelibrary.com]