Table 1:

Candidate stripping techniques tested on Stage 1.

Strategy	Description	Reference for protocol
Heat	Microwave in citrate buffer with 0.05% tween. High setting until boiling then 15 minutes at 20% (Note: referenced paper suggests 5 minutes at 50%).	Toth and Mezey (2007)
Glycine/SDS at low pH	Glycine mixed with SDS, at pH 2.0 and heated to 50°C for 30 minutes.	Pirici et al. (2009)
Denaturing solution	One part of solution A to two parts of solution B from the Denaturing Solution Kit from Biocare Medical	Manufacturer instructions (part DNS001L)
BME	Tris-HCl mixed with SDS and BME, heated to 56C and incubated for 30 minutes. Washed in TBS.	Gendusa et al. (2014)