Table 5.

Repeat CRPS patient serum (n=10) testing in muMT fracture mice

CRPS Patient ID	CRPS Persists	Interval BetweenTests (m)	Injury Duration (months)	Sex	Age	CRPS Injury	Allodynia	Edema	NRS	3 wk post FX mice		FX mice 7d post serum injection
										Allodynia (Δg)	Unweighting (%)	Allodynia (Δg)	(%Δ)	Unweighting (%)	(%Δ)
WK-1	Yes	31	38	F	55	Hand FX	Yes	Yes	7	−0.7	14	−0.6	−12	13	−13
WK-2	No	32	33	F	56	CTS SX	No	No	0	−0.9	17	−0.9	7	14	−18
WK-7	Yes	29	53	F	48	Ankle Injury	Yes	Yes	7	−0.8	15	−0.7	−17	15	0
WK-11	No	27	29	F	60	Hand FX	No	No	5	−0.8	15	−0.7	−14	17	12
Gi-1	Yes	41	56	F	56	Hand SX	Yes	Yes	4	−0.9	15	−0.8	−11	15	0
Gi-3	No	33	35	F	51	CTS SX	No	Yes	3	−0.9	14	−0.7	−26	15	7
Gi-6	Yes	31	42	F	46	Hand FX	No	Yes	9	−0.9	14	−0.8	−2	16	14
Gi-9	No	18	21	M	67	Hand SX	No	No	0	−0.9	14	−0.9	0	15	10
Gi-12	No	33	37	F	52	Hand Injury	No	No	6	−0.9	14	−0.6	−28	13	−7
Gi-13	Yes	15	17	F	57	Hand FX	Yes	Yes	4	−0.9	16	−0.8	−18	15	−7

All 10 patients initially met the IASP CRPS diagnostic criteria at the time of first serum collection and all the initially collected serums were pronociceptive when injected into fracture muMT mice lacking B cells and immunglobulin. When repeat serum was collected at a later time point from these same patients (average interval between collections was 28.8 ± 2.7 months), none of the repeat serums had pronociceptive effects in the fracture mice. At the time the repeat serum was collected 50% of the patients previously diagnosed with CRPS no longer met the IASP CRPS criteria (WK-2, WK-11,Gi-3, Gi-9, Gi-13), the average time elapsed since injury was 35.9 ± 4.4 months, the average age was 54.8 ± 2.1 years, and the average pain numerical rating score (NRS) was 4.2 ± 1.0. Patients with persistent CRPS are labeled in yellow. Allodynia was present in 40% of patients, limb edema was present in 60% of patients, and 40% had suffered a fracture. All FX mice had allodynia and unweighting in the injured hindlimb at 3 weeks post FX. The average change in FX mouse hindpaw allodynia at 7 days (7d) after control subject serum intraperitoneal injection was −12 ± 4% and the change in hindlimb unweighting was 1 ± 4%. Post injection changes in allodynia or unweighting that reached the 25% increase significance criteria are listed in Bold font. FX: fracture, (Δg): difference between contralateral hindpaw threshold and fracture hindpaw threshold, thus a negative value represents increased allodynia, (% Δ): % change between 3 wk post FX value and 7d post serum injection value, thus a positive value represents an increase in allodynia or unweighting after serum injection.