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. 2020 Jun 3;15(6):e0233887. doi: 10.1371/journal.pone.0233887

Fig 1. Status of genetic alterations, AKT/mTOR pathway, and LDs across normal and malignant renal cell lines.

Fig 1

(A) Copy number alterations (CNA) across three malignant ccRCC cell lines (769-P, 786-O, A-498) and mutational status (Mut) derived from TCGA. (B) Western blot analyses of ccRCC cell lines and HK-2 cells with the indicated antibodies. Three independent experiments were performed and representative blots are displayed. (C) LD assessment of ccRCC cell lines and HK-2 cells via immunofluorescence staining using LipidTOX. Three independent experiments were performed and representative images are displayed. (D) Real-time PCR analyses of genes involved in LD biogenesis and regulation in ccRCC cell lines and HK-2 cells. Data shown is the composite of three independent experiments.