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. 2020 Jun 3;15(6):e0233887. doi: 10.1371/journal.pone.0233887

Fig 6. LPA antagonizes TEMS-induced mitochondrial alterations in a malignant renal cell line.

Fig 6

(A) Real-time PCR analyses of ATX in ccRCC cell lines and HK-2 cells in response to 24 hour treatment with 10 μM TEMS. Data shown is the composite of three independent experiments. Left panel, normalized to each cell line (untreated). Right panel, normalized to HK-2 cells (untreated). (B) Western blot analyses of A-498 cells (grown in CM or SFM media conditions) treated with 10 μM TEMS in the absence or presence of 10 μM LPA with the indicated antibodies. Three independent experiments were performed and representative blots are displayed. (C) Mitochondrial network assessment of A-498 cells in response to 24 hour treatment with 10 μM TEMS with/without 10 μM LPA via immunofluorescence staining of TOM20. Three independent experiments were performed and representative images are displayed. (D) Mitochondrial network assessment of A-498 cells in response to 24 hour treatment with 10 μM TEMS with/without 10 μM LPA via immunofluorescence staining of TOM70. Three independent experiments were performed and representative images are displayed.