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Conventional tissue fixation and processing method [Figure 2]
| Steps | Reagent/processing fluid | Time |
|---|---|---|
| Dehydration | 70% isopropyl alcohol | 30 min |
| 80% isopropyl alcohol | 30 min | |
| 95% isopropyl alcohol | 30 min | |
| Absolute alcohol-I | 45 min | |
| Absolute alcohol-II | 45 min | |
| Clearing | Xylene-I | 30 min |
| Xylene-II | 30 min | |
| Xylene-III | 30 min | |
| Impregnatio n | Paraffin wax-I | I h |
| Paraffin wax-II | 1 h | |
| Paraffin wax-III | 1 h | |
| Total time | 450 min |
