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Schedule for H & E staining by conventional method
| Reagent | Time |
|---|---|
| Xylene-1 | 10 min |
| Xylene-II | 10 min |
| 95% isopropyl alcohol | 1 dip |
| Absolute alcohol | 1 dip |
| Water bath | 5 min |
| Hematoxylin | 7-10 min |
| Water bath | 10 min |
| 1% acid alcohol | 1 dip |
| Water bath | 10 min |
| Eosin | 1 dip |
| 95% isopropyl alcohol | 1 dip |
| Absolute alcohol | 1 dip |
| Xylene | 10 min |
